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文献和实验sit for 2 hours. Transfer worms with a Pasteur pipet in as little liquid as possible to a feeding plate. Allow the plates to sit at room temperature (RT) or at 20°C for 30 minutes to 2 hours to pass the dye out of the digestive tract
FREEZE-CRACK b-GALACTOSIDASE STAINING PROTOCOL
uls of stain mix on each well. Be sure to completely cover the well. 19. Let develop for 12 hours. Store the slide in a humidity chamber at RT or in the refrigerator. 20. When ready to view slides under the light microscope, remove excess staining
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
pK for 15 min at room temperature. 4. Wash in deionized water (4x2 min). 5. Cover sections with 2% H2 O2 for 5 min at room temperature, to inactivate endogenous peroxidase. 6. Wash in deionized water. 7. Immerse in TdT buffer.
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