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文献和实验bath for 3 min. 8.Add 100 µl 2x Laemmli sample buffer(or use 75μl of 3X Laemmli sample buffer). 9.Place in boiling water bath for 1 min. 10.Spin briefly in Tomy (at room temp--15 sec,3K),remove liquid with a ml pippetteman tip,and transfer to 1.5 ml
Protein Expression and Purification of MBP-T antigen (131-259)
thrombin (200U for 6 L prep.)and dialyze sample against buffer CO/N in cold room.Make sure the cleavage is complete.If it is not complete let the reaction go at room temperature with another addition of 100μl of thrombin. xvi.Pass the sample over pre
inoculation of 3 ml of antibiotic containing media with the plasmid or cosmid-containing bacterial colony, and then increasing the culture volume to 50 ml, and then to 4 l. However, it was observed that recombinant cosmid DNA isolated from cell cultures grown
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