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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
.edu phone: 405 325-4912 fax: 405 325-7762 August 5, 1995 A printed version of this protocol book can be obtained from: "DNA Isolation and Sequencing" (Essential Techniques Series) by Bruce A. Roe, Judy S. Crabtree and Akbar S. Khan Published
In a separate 96-well plate, prepare a titration series of the biotin-labeled ZO-1 and the unlabeled ZO-1, XH1, XH2, and control PC10 antibodies over five 10-fold dilutions using PBS/BSA (1%) as a diluent. The starting concentration of the antibody solutions
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