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文献和实验you want). See "Plasmid DNA Preparation" protocols for detailed information. Each TA muscle is injected with 50 µl of DNA solution. To inject plasmid DNA use a 27GX3/4" (0.4x20mm) needle attached to a 1 ml tuberculin syringe. A piece of polyethylene
µg of protein). This sample is a bit viscous, and is best loaded with a Hamilton syringe: I use the type that holds 50 µl and has a 22 g needle cemented permanantly on. If you are running a Western on E. coli expressing a high abundance (fusion
MBP in kinase buffer. 12.) Wash the immunoprecipitate 3x with RIPA buffer and once with kinase buffer. 13.) Add 40 μl of the reaction mixture, along with 15 μCi of gamma-32P-ATP (3000Ci/mmol, NEN) to the washed beads and mix very gently
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