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文献和实验In Planta Transformation of Arabidopsis
(or equivalent) Cover for plants, transparent Dessicator with vacuum pump and glass dish (optional, for Steps 13-16) Incubator, preset to 28°C Spray bottle (optional, for Steps 17-19) Standard equipment for growing Arabidopsis (see Step
(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR
Studying Wound Repair in the Mouse
scissors and forceps Disposable biopsy punch, 5 mm diameter (Stiefel; http://www.stiefel.com; optional) Tegaderm transparent
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