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文献和实验Fast DNA Fragment Sizing in a Short Capillary Column
time per sample. This has been accomplished by using a range of an effective length capillary as short as 7 cm and field strength as high as 2000 V/cm for the separations of small ions, drugs, and proteins (4 -7 ).
7.5. 3. 10% (w/v) NaN3 . 4. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.3. Fractionation of Wnt3A CM 2.3.1 Step 1: Blue Sepharose 1. Sample: 1–4 L Wnt3A CM as prepared in Section 2.2 . 2. Column: Blue Sepharose HP
. Rinse with distilled water; double distilled water and ethanol. Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder
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