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文献和实验so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
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