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文献和实验Bacterial Efflux Pump Inhibitors
. One of the major antibiotic resistance mechanisms utilized by more than 15 species of Gram-negative bacterial cells is the Resistance Nodulation Division (RND) efflux pump, which eliminates several classes of antibiotics such as penicillins and cephalosporin macrolides
Simulation Studies of the Mechanism of Membrane Transporters
. Furthermore, in order to prevent the formation of leaky states and to be able to pump the substrate against its electrochemical gradient, all membrane transporters use the widely-accepted “alternating access mechanism,” which ensures that the substrate
Chick Chorioallantoic Membrane (CAM) Assay
", OD 3/32", wall 1/32") to a 3cc syringe with a 20-gauge needle. The syringe was attached to an infusion pump (Harvard Apparatus, South Natick, MA). Injection of 400 μl FITC dextran, MW 2,000,000 (Sigma, St. Louis, MO), into the umbilical vein
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