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文献和实验7.5. 3. 10% (w/v) NaN3 . 4. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.3. Fractionation of Wnt3A CM 2.3.1 Step 1: Blue Sepharose 1. Sample: 1–4 L Wnt3A CM as prepared in Section 2.2 . 2. Column: Blue Sepharose HP
RADIOLABELING OF PROBES FOR ELECTROPHORETIC MOBILITY SHIFT ASSAYS
cc luer lok syringe without the barrel.Insert the barrel,and push the reaction mixture through the column SLOWLY (this should take approx.45 sec.) unscrew the syringe,remove the barrel.Add 75μl of fresh STE to the top of the column and repeat step 10
free shotgun sequencing library from miniprep BAC DNA
buffer pH 8.0 or ddH2O. 2.NotI restriction digestion 3.265 μl miniprep DNA 4.30 μl of 10X New England BioLabs Buffer 3 5.5 μl of 10 units/μlNot 1 (New England BioLabs, #189) 6.incubate for 1hr at 37℃. 7.Purify insert DNA with PFGE Add 60 μl of 6X
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