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文献和实验Large Scale Plasmid Preps: Qiagen/Cesium Method
transfections of mammalian cells. PROCEDURE: Grow 250 - 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4ºC for 10 min. Purify the plasmid
Marcantonio Lab Protocol Manual
during the termination reactions. Transparent tape should be applied over any wells that will not be needed, in order to provide a better seal against water leaking into the sample wells. The horizontal rows should be labelled A-T-G and C, while the vertical columns
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