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文献和实验1.5 (bromoacetylation) and 1.6 (displacement). 8) After the final displacement is done, rinse with 2 mL of DMF (repeat 4x), then 2 mL of dichloromethane (repeat 3x). Cap and store the reaction vessel until cleavage. 9) Pause in synthesis
, or by adsorption to glass as in the commercial Qiex kit, and this will increase the efficiency of labelling considerably. - Boil the DNA to denature it for a few minutes. Briefly spin the tube and put on ice to cool. Add 6.6 µl 5X OLB, 5 µl hot dATP (50 µCi
will be detected, and thus it is important to achieve good separation of fragments in this range. Typical gel conditions: use a 35 cm long x 15 cm wide gel box. The gel poured inside is 23 cm long. Pour a 0.8% agarose gel in 1X TAE, with EtBR in the gel
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