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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
tubes (6 mm ID x 50 mm). Washes are done by low-speed spins and all solutions (except fixatives) contain 0.1% Tween 20 to prevent gonads from sticking to glass. The key to making this work consistently is to fix dissected gonads in a combination
AFLP: not only for fingerprinting, but for positional cloning
) SDS 10 mM ß-mercaptoethanol (add just before use) 3) Add 150 µL 5 M K-acetate, vortex, incubate on ice for 20 min. Spin @ 12K x g in microfuge for 10 min. 4) Transfer 750-800 µL supernatant to new microfuge tube and add an equal vol
AFLP: not only for fingerprinting, but for positional cloning
. Extraction Buffer 50 mM Tris, pH 8.0 10 mM EDTA, pH 8.0 100 mM NaCl 1.0 % (w/v) SDS 10 mM ß-mercaptoethanol (add just before use) 3) Add 150 µL 5 M K-acetate, vortex, incubate on ice for 20 min. Spin @ 12K x g
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