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文献和实验Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS
of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC
Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting
, such a global approach allows attaining the most discriminatory protein biomarkers that can best predict the disease status. In this chapter, we describe the protocols for proteomic analysis of saliva using 2D gel electrophoresis, Western blotting, and LC-MS/MS.
with microscale solution isoelectric focusing (MicroSol IEF). MicroSol IEF is compatible with most downstream proteome analysis methods including narrow range 2-D gels and 1-D gels followed by LC-MS/MS or LC/LC-MS/MS. This chapter describes the use of MicroSol IEF
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