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文献和实验Streptomyces:Protocols/Spore Prep
the suspension into the falcon tube by replacing the syringes’ plunger. Spin the falcon @ ~5000g for 5 minutes. Re-suspend the pellet in minimum volume necessary of sterile 20% glycerol (~500μl dependent on the pellet size). Transfer the spore
by combining a small amount of a Wnt samplewith 4× loading dye and water to give a final concentrationof the loading dye of 1× in a total volume of 20 to 40μL. Use the following amounts of Wnt per 20 μL sample: 1)Wnt3A CM: 1 μL; 2) Blue Sepharose fractions: 1 μ
1. Run samples out on a gel. For bacterially expressed proteins, generally 5 μl is plenty (1ml cell cμlture; cells resuspeded in 50 μl loading buffer). Run the gels (BioRad mini gels) at 195 V for approximately 40 min. (until samples run close
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