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文献和实验In-gel Tryptic Digest for Protein ID by Mass Spectrometry
to 12.5 ng/μl.10.Remove trypsin-containing buffer.Add5-10 μl 50 mM NH4HCO3 without trypsin to keep pieces wet during cleavage.Incubate o/n at 37℃.11.Spin 1' at 14,000 rpm to spin down gel pieces.Save supernatent in a separate PCR tube.12.Add 20 μl 20 mM
Using Cell‐ID 1.4 with R for Microscope‐Based Cytometry
of yeast and mammalian cells processed by Cell‐ID. (A ) From left to right: yeast in focus, slightly defocused (note the dark ring on the border of the cells), and the same cells after Cell‐ID has identified each one and traced their borders correctly
In Situ Hybridization Using Digoxigenin Labeled Probes
as black bands. Weaker signals are brown Solutions: 1) 10X PBS 1.3 M NaCl 0.07 M Na2HPO4 0.03 M NaH2PO4 2) Hybridization Buffer 0.6 M NaCl 50 mM Sodium phosphate buffer, pH 6.8 1X Denhardt's (0.02% BSA / 0.02% Ficoll / 0.02
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