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文献和实验In Vivo Luciferin Imaging Procedure
the quantity of needed for an individual experiment. • Syringe filter, 0.2 μM Procedure 1. Prepare a fresh stock solution of Luciferin at 15 mg/mL or 30 mg/kg in DPBS. Filter sterilize through a 0.2 μM filter. 2. Inject 10 μL/g of body weight
Construction and Characterization of Adenovirus Vectors
visible on the gradient and will be found at the interface between the 1.25- and 1.35-g/mL layers of the gradient. 40. Use a 22-gauge needle attached to a 3-cc syringe to pierce the tube ~1 cm below the virus band. Turn the bevel
Tamoxifen administration to mice
behind the tongue (max. 1 cm). It is helpful to mark 1 cm on the needle with an edding pen in order to see how deep you have inserted the needle. slowly introduce 50 μl of the tamoxifen solution with the syringe; assure swallowing; e.g. repeat
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