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文献和实验Introduction Basic Protocol 1: Single Labeling of Actively Proliferating Cells to Analyze the Short‐Term Effect of a Soluble Factor on aNSCs Basic Protocol 2: Dual Labeling of Two Cell Division
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
to live cells which remain 7-AAD-. Late apoptotic or necrotic cells which have lost membrane integrity appear 7-AADbright . Reference : Schmid I, Uittenbogaart CH, Keld B, and Giorgi JV: A rapid method for measuring apoptosis and dual-color
these procedures to yield direct single cell expression information. Fluorescence in situ hybridization (FISH) is the gold standard for localization of nucleic acids (Fauth and Speicher, 2001; van der Ploeg, 2000). The introduction of amino-allyl modified bases
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