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文献和实验相关专题 Transformation-Competent E. coli preparation Rubidium chloride method Inoue "ultra-competent" method Cosmid packaging protocol DNA Ligation and Transformation Protocols CONTENT
Construction and Characterization of Adenovirus Vectors
. Once the viscosity of the lysate is near that of water, centrifuge at 1000g for 10 min at 22°C. 38. Prepare CsCl step gradients in Ultra-Clear ultracentrifuge tubes (two tubes per virus). i. Add 2 mL of 1.35 g/mL CsCl
DNA Extraction Promega DNA-IQ System Database Protocol
or pair of tweezers Container of sterile 1.5 mL microcentrifuge tubes DNA-IQ magnetic stand Biohazard waste container Set of micropipettors One small beaker for waste Calculator Timer Ultra-fine point sharpie
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