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文献和实验on the stage, cover slip up, of course. Use dark field microscopy to find the focal plane at 40x using the Vaseline film or an air bubble as a target. Switch to 100x, still in dark field mode, and you should be able to focus on high contrast objects
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
, using 400x in phase contrast mode. Can you see one or more flagella? Add a drop or two of Lugol's iodine to what remains in the culture tube (ratio should be 4:1 culture to iodine). Mix and prepare a slide of one drop of culture, with cover slip
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
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