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文献和实验Inhibition of the fast and slow blocks to polyspermy
Procedure 1a) To inhibit the fast block to polyspermy, resuspend eggs in ASW or in nicotine (Sigma Chemical Company) at 0.25 mM, 0.5 mM and 1 mM, (dilute 1.25 ul, 2.5 ul and 5 ul of a 40% solution of nicotine free base in 10 ml ASW and use
Mag-Binds Plant DNA Kit Magnetic Fast Protocol
preset at 65-70°C 4. Equipment for disrupting plant tissue (MM300 Mixer Mill or Geno/Grinder 2000) 实验步骤 1. Collect plant tissue (start with 50mg for fresh sample and 10mg for dried samples) in a 1.2 ml 96 well plate
Inhibition of the fast and slow blocks to polyspermy
Procedure 1a) To inhibit the fast block to polyspermy, resuspend eggs in ASW or in nicotine (Sigma Chemical Company) at 0.25 mM, 0.5 mM and 1 mM, (dilute 1.25 ul, 2.5 ul and 5 ul of a 40% solution of nicotine free base in 10 ml ASW and use
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