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文献和实验7.5. 3. 10% (w/v) NaN3 . 4. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.3. Fractionation of Wnt3A CM 2.3.1 Step 1: Blue Sepharose 1. Sample: 1–4 L Wnt3A CM as prepared in Section 2.2 . 2. Column: Blue Sepharose HP
v468 Chapter 9 自身启动子报告基因验证转录靶点
of the reaction mix to electrophoresis on a 1%(w/v) agarose gel to check for correct size of the amplicon. 4. For subsequent purification of the amplicon from the reactionmix, use the Qiagen PCR Purification Kit according tothe user manual, and elute in 30 μL
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
supply. 2. Pre-poured SDS-polyacrylamide mini-gels (Bio-Rad or Invitrogen);store refrigerated. 3. Running buffer: 25 mM Tris, 192 mM glycine, and 0.1 %(w/v) SDS. Store at room temperature. 4. Pre-stained molecular mass markers: Kaleidoscope
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