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文献和实验Simplified Arabidopsis Transformation Protocol
. Grow under long days in pots in soil covered with bridal veil, window screen or cheesecloth.2.(optional) Clip first bolts to encourage proliferation of many secondary bolts. Plants will be ready roughly 4-6 days after clipping. Clipping can be repeated
Simplified Arabidopsis Transformation Protocol
from Agrobacterium-treated plants). 1.Grow healthy Arabidopsis plants until they are flowering. Grow under long days in pots in soil covered with bridal veil, window screen or cheesecloth. 2.(optional) Clip first bolts to encourage
containing antibiotics until use. 2) Prepare 1 % agarose-coated operating dish , rinse with HBSt and antibiotics. 3)Transfer donor and recipient embryos into operating dish containing 1X HBSt and antibiotics 4) Dip all tools into 70% EtOH
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