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文献和实验screws, 1 pair of tweezers, one 75 x 25 x 1 mm glass slide (or other desired cell surface material), and 2 surgical towels. 6) Place sterile towels into laminar flow hood. Place the flow circuit, flow chamber, 4 x 4-way stopcocks, 1 x 1-way
Isolation of micro-RNA (miRNA)
water equal to 1/2 of volume D. Mix vigorously. 17. Accurate spectrophotometric measurement of RNA with 500-μl quartz cuvettes require at least 1 μg of RNA. To quantify the RNA, remove a small sample and dilute it with a 5 mM Tris-HCl, pH
Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
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