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文献和实验of EPC medium x 6 hr if a confluent cell layer is desired at the initiation of flow (Fig. 11A). 2) In order to test cell spreading and adhesion under fluid shear stress, allow 3 x 106 cells to adhere for only 15 min before commencement
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
for 30 minutes at room temperature.6. Wash two times in PBS and resuspend cell pellet in PBS. Cells may be stored at 4℃ for up to 8 hours.7. Stain activated platelets by direct immunofluorescence using CD62P-FITC, catalog number 555523 (31794×), at 20 μL
Indirect flow cytometry (FACS) protocol
and resuspend them in ice cold PBS, 3% BSA, 1% sodium azide. 11. Store the cell suspension immediately at 4°C in the dark. 12. Analysis: For best results, analyze the cells on the flow cytometer as soon as possible. We recommend analysis
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