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上海希言科学仪器有限公司
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文献和实验Isolation of micro-RNA (miRNA)
water equal to 1/2 of volume D. Mix vigorously. 17. Accurate spectrophotometric measurement of RNA with 500-μl quartz cuvettes require at least 1 μg of RNA. To quantify the RNA, remove a small sample and dilute it with a 5 mM Tris-HCl, pH
A Method for Micro-Scale Isolation and Purificatio
caps (#8142) Protocol: Phases: Organic : 2 volumes DIPE/1-butanol, 60:40 v/v Aqueous : 1 volume saline solution Aqueous phases are as follows: Cell pellets : for cells, use 0.l% NaCl: 10 ml saline
A Method for Micro-Scale Isolation and Purification of Gangliosides
Protocol: Phases: Organic : 2 volumes DIPE/1-butanol, 60:40 v/v Aqueous : 1 volume saline solution Aqueous phases are as follows: Cell pellets : for cells, use 0.l% NaCl: 10 ml saline per total lipid extract of 109 cells
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