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文献和实验Construction and Characterization of Adenovirus Vectors
2006 ). Suspend the DNA in a total volume of 25 µL of 0.1X TE-Ad. 12. Repeat Steps 5-10 with 5 µL of DNA (from Step 11) and 100 µL of DH5 cells, rather than BJ5183. 13. Select two colonies from each plate. Purify the DNA
, which it is an efficient and easy to use PC control equipment that allowed to cast simultaneously 10 to 60 gels. Gel composition and dimension Dimension: 160 x 200 x 1.5 mm Resolving gel: Acrylamide/PDA (9-16% T / 2.6% C) Stacking
(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
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