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文献和实验Construction and Characterization of Adenovirus Vectors
, purification, and characterization of first-generation Ad vectors. RELATED INFORMATION Figure 1 presents a schematic representation of the AdEasy system for Ad vector production. The shuttle, containing the transgene of interest, is produced
E-Z 96 Plant DNA Protocol-Vacuum Manifold Processing
Note: The following protocol is based on using OBI’s vacuum manifold (Product No. VAC-03). 1. Set up vacuum manifold by following manufacturer’s instructions. 2. Place waste collection tray inside the vacuum manifold, then place
Polymerase III in vitro Transcription
Polymerase III in vitro Transcription Steve Hahn For the following reactions, use appropriate shielding and dispose of radioactive waste properly! A 20 microliter transcription reaction contains: 4.0 μl 5X Pol III transcription buffer 0.2 μl
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