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文献和实验to the capillary when it is pulled out. If this occurs, the capillary should be discarded. The correct injection volume of the Transjector is set by defining the injection time and the injection volume for all embryos. Femtotips and Microloaders Femtotips
Embryo Dissection and Micromanipulation Tools
the wax with a warmed pipette tip. Once set, the wax holds the loop in place. A hair loop can also be set in beeswax by dipping it into molten beeswax (melted on a hotplate). After removing the loop from the wax, blot excess wax from the loop
Single Primer ("Semi-Random") P
Distribute 3 10 µL aliquots of each mix into capillaries and discard remainder. Load sets of 3 capillaries, one from each mix, consecutively into the same machine with the annealing temperature ("Ta") set at 40, 45 and 50°, respectively. Cycle as follows
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