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文献和实验Marcantonio Lab Protocol Manual
laterally. 4) Gradually tighten the screw knobs, in an alternating manner, to complete the seal. Be careful not to overtighten the screws as this may damage the plates. 5) Close the upper buffer drain valve and fill the upper and lower reservoirs
from powder) in Buffer A (dissolve the p-formaldehyde by putting the solution in boiling water bath for few minutes). Use this solution to transfer the embryos to a glass flask, or transfer the embryos with a paintbrush into a vial that already contains
without hurting the eyes. Adjust the condenser To adjust and align the microscope, start by reading the manual. If no manual is available, try using these guidelines. If the condenser is focusable, position it with the lens as close to the opening
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