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文献和实验into a separate PCR tube and set aside. TIP: DNA that is recovered from microdissected samples and "semi-purified" using a one-step proteinase K buffer will sometimes produce "non-specific" PCR products in addition to the true alleles. Moreover, larger alleles
the CRC filter (35-µm) into the CRC. Place the CRC in a standard microcentrifuge tube. Add ~30 µL of the Ni-NTA resin slurry to the bottom of the CRC tube. Drain the column by centrifugation at 120g for 1 min at room temperature. These centrifugation
(4heads/10ul) 4. Spin at 112000 rpm in an Eppendorf microfuge for 10 min at 4°C. 5. Transfer the supernatant to a new tube and discard the pellet. 6. Boil for 3 min at 99°C. 7. store at 4°C. B. Polyacrylamide gel 1. Resolving gel: 10 ml
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