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文献和实验Modified protocol for Isolating RNA from Grape Leaves
实验步骤 1. Ground sample with liquid nitrogen. 2. Weight 10-50 mg ground sample into a tube contains 400 ul CPL Buffer. Mix the sample throughly by vortexing the tube 20 seconds. 3. Add 400 ul chloroform and mix
Methods for the Detection of D-Amino-Acid Oxidase
could be done in a single tube using Titan One Tube RT-PCR System (Boehringer Mannheim) or One Step RNA PCR Kit (Takara). Both gave satisfactory results. Total RNA was extracted from organs, tissues, or tissue culture cells as described above. Poly(A)+ RNA was purified
Methods for the Detection of D-Amino-Acid Oxidase
and PCR were carried out separately. However, these two steps could be done in a single tube using Titan One Tube RT-PCR System (Boehringer Mannheim) or One Step RNA PCR Kit (Takara). Both gave satisfactory results. Total RNA was extracted from organs
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