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文献和实验solution). Boil 3-5 min aand keep on ice immediately. Load 1 µl(Qiagen prep) - 2 µl (AutoGen prep) High resolution denaturing gel (Stratagene precast sequencing gel (cat.# 401092) Loading pattern: N M S
enough, repeat the procedure.Fingerprinting GelEquipment and materials:Stratagene sequencer - CastAwayTM gel system with gasket lubricant(Cat # 401075)Stratagene castAwaytM precast gels (Cat # 401092): 4.5% precast polyacrylamide, 1xTBE, 7 M urea, with 32
purch asing a sequencing kit, we use either the T7 Sequencing Kit (Pharmacia, 100 reactions) or the Sequenase 2.0 Sequencing Kit (USB via Amersham in the U.K., 100 reactions). Reactions are performed in sterile 1.5ml microcentrifuge tubes. Primers
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