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文献和实验of gel or urea is left. Stand the gel in the lower buffer reservoir so that the notched glass plate faces the top reservoir (make sure the seal is in it’s groove) and the metal plate in the back. Clamp the gel with the metal plate
hybridization chamber, add 5 µl of 3x SSC in the reservoir, if the chamber provides one, or at the scribed end of the slide and seal the chamber. Submerge the chamber in a 65°C water bath and allow the slide to hybridize for 16-20 hours. There are a wide
Marcantonio Lab Protocol Manual
, move the plates to a more vertical position and tap the glass plates to work the bubbles out. 4) Pour gel solution until there is a reservoir of liquid extruding from between the plates when they are resting at a 5 degree angle. 5) Insert the flat edge
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