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文献和实验Micropipet Aspiration for Measuring Elastic Properties of Lipid Bilayers
bilayered vesicles are aspirated into a pipet of inner diameter ≈8 �m. The changes in projection length of each vesicle inside of the pipet in response to changes in aspiration (suction) pressure are used to determine mechanical moduli. The suction pressure
PREPARATION OF MICROINJECTION PIPETTES
INJECTION AND HOLDING PIPETTES The glass capillary tubing used should be thin walled, borosilicate glass without a fibre. e.g. Clark Electromedical Instruments GC 100T - 15 (= 15 cm) GC 100T - 10 (= 10 cm)
Preparation of Microinjection Pipettes
when touched with the edge of the scalpel blade. The resultant pipette should have a relatively long section at the appropriate dimater (18-20 µm). Holding pipettes Holding pipettes are blunt flame-polished pipettes through which suction is applied
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