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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
either by Quiaquick PCR purification kit or by phenol:chloroform followed by ethanol precipitation. 5) DIGEST LIGATION PRODUCTS WITH NDE I 20 ul 10 X NEB buffer 4 ul phenol-extracted, ligated DNA - volume is variable 5 ul Nde I enzyme (100 U) 200 ul final
Studies of the Ubiquitin Proteasome System
and Translation Systems In Vitro Production of Ubiquitin‐Protein Ligases (E3s) Basic Protocol 24: Expression of Ring‐Finger E3s in Bacterial Lysates Alternate Protocol 14: Expression of HECT and U‐Box E3s in Bacterial Lysates Alternate Protocol 15: Expression
Practical Methods for Molecular In Vivo Optical Imaging
medium Insert DNA plasmid (e.g., from an Endo‐free midi or maxi‐prep kit) Packaging DNA plasmids
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