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文献和实验Dichlorodimethylsilane Sigma Aldrich 80430-500G-F For siliconization of glass reaction vessels Disposable PP fritted cartridge
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
as this may break cells, this is especially important in step G. E. Add 10 ml of pre-warmed hypotonic solution colcemid working solution to each tube. Seal tightly and invert to mix. Incubate the tubes at 37 C for 15 minutes. F. Centrifuge for 6 minutes
Single and Double FISH protocols for Drosophila
to the bottom of the tube (1-3hr or overnight at 4oC) before resuspending and mounting. 10. Transfer the embryos to a clean slide in ~ 80 ul mountant and cover with a 25 X 50 mm coverslip. Seal the edges with nail polish. Slides can be stored for weeks at 4oC
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