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上海希言科学仪器有限公司
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文献和实验Northern Blotting方法(Howell Lab)
inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube
Recovering Plasmids from your Stab Culture
-term storage is unknown. Most labs store bacteria in 15-25% glycerol. As an example, a 25% glycerol stock can be made by adding 500 μL of an overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube. Freeze the glycerol stock tube at -80o C
Streptomyces:Protocols/Spore Prep
tube(s) (MCT), 2mL screw cap tube(s), 50mL falcon tube(s), sterile 10mL syringe, filtered tips, forceps (tweezers), laminar flow hood, vortexer, 30°C incubator, microwave, falcon centrifuge. Preparation Ensure the equipment and sterile reagents
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