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文献和实验The Activity of rRNA Resistance Methyltransferases Assessed by MALDI Mass Spectrometry
of bacteria and differ in whether they add one or two methyl groups to the A2058 target. The dimethylated rRNA confers a more extensive MLSB resistance phenotype. We describe here a method using matrix-assisted laser desorption/ionization (MALDI) mass
Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry
Peptide mass fingerprinting (PMF) by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides a simple and direct means to unequivocally confirm identity of recombinant proteins based on predicted
target for MALDI-MS analysis. Resume the carboxypeptidase Y reaction by repeating Steps 16-19. Mass Spectrometry Perform MALDI analyses on the samples (from Steps 11, 14, and 20) using a delayed-extraction TOF mass spectrometer. TROUBLESHOOTING
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