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文献和实验BAC DNA Purification For Injection
This is a two step procedure, utilizing a Qiagen Large-Construct Kit for the initial purification, followed by either a chromatographic separation OR gel purification. Qiagen Prep of BAC DNA Modified from Biotechniques 27:72-74 (1999
Pronuclear injection to produce transgenic mice
like is appropriate. You can dialyze overnight against 2 liters of injection buffer at this point, but it's really not necessary. This method of prepping fragments is proving to be a really reliable and clean method. I have used it on pieces as large as 18 kB
Separation of Dna by Capillary Electrophoresis
. The various techniques involve electrokinetic injection of the DNA onto a narrow band at the head of the column, sieving the DNA through various entangled polymer matrices, and detection via single or multichannel laser-induced fluorescence. Analytical
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