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文献和实验on the bottom plate of the flow chamber. Make sure that the slide is placed with the cell-seeded side facing up. 4) Pipet 10 ml of warm EPC medium onto the slide. Allow the medium to cover the slide and flow chamber, but do not spill the medium
Blood & Body Fluid DNA Protocol
the lysate from step 4 into the column, and centrifuge at 10,000 x g for 1 min to bind DNA. Discard the collection tube and flow-through liquid. 7. Place the HiBind® DNA Mini Column into a NEW provided 2 ml collection tube. Add 500μl of Buffer HB
Flow Cytometric Analysis Cell Cycle
sensitive) and incubate at room temperature for 5-10 minutes.9) Place samples in 12 X 75 Falcon tubes and read on Becton Dickinson FACStarPLUS.ReferenceExperimental Cell Research 207,142-151.
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