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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
centrifugation (3000-5000 x g (6600 rpm) for 1 min)), and remove supernatant (use gel loading tips to completely remove supernatant). (23) Add 1 ml of ice-cold Low Salt Immune Complex Wash Buffer (EZ ChIP kit). Resuspend the packed agarose beads by gentle
Endothelial Cell Systems - Instructions For Use
. The cryopreserved cultures (HUVEC primary 1・ - all others tertiary 3・) are shipped in a screw cap cryovial containing approximately 500,000 cells. A Certificate of Analysis is provided with each cell strain and indicates date of cryopreservation. QC performance
for 1 minute. F. IP Elution 1. Re-fit column plug, add 150μL 1X IP Elution Buffer to the washed resin, cap the column, and incubate at 65°C for 30 minutes with shaking. If a thermomixer is unavailable, incubate the column in a heat block set a 65°C
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