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文献和实验线虫蛋白提取--Making Protein Gel Samples from Worms
for other strains to catch up. 6A. If you don't need embryos in your sample, can just add 1 ml gel sample buffer and boil the tube (use a screw cap eppendorf) for 5 minutes, spin the tube 10 min. to pellet debris, and transfer the supernatant to a new tube
with syringe. Un-clamp gel. Pry apart glass plates with spatula. Cut off upper right corner of gel. Place saran wrap on top of gel, flip over and remove other glass plate. (Be sure to wet the gel with a little TBE so it does not dry out and break
Procedures for In Vitro Production of Bovine Embryos
is a lot but some of these tubes of HEPES-TALP will be used later in the day.2. Add 10 ml Sp-TALP to a 15 ml conical tube. Tighten the cap and place in the incubator.3. Add 5 ml IVF-TALP to a 15 ml conical tube. Leave cap loose and place in the incubator
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