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文献和实验time of 14 days based on their cobblestone morphology. Once EPCs in culture cover ¼ of the 12-well surface area, expand cells and confirm EPC identity with flow cytometry by testing for presence of surface markers CD31 and absence of CD14, CD
Using Drosophila S2 Cells to Measure S phase-Coupled Protein Destruction via Flow Cytometry
DNA replication with ubiquitylation and subsequent proteolysis by the 26S proteasome. Here we describe a flow cytometry-based method using Drosophila S2 cells that recapitulates S phase-specific protein proteolysis. Because of the high degree
Micro-CT Technique for Three-Dimensional Visualization of Human Stem Cells
Micro-CT offers high spatial resolution of the distribution of stem cells and provides rapid reconstruction of 3D images and quantitative volumetric analysis. Together with real-time PCR analysis, micro-CT offers the possibility to obtain
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