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文献和实验oligo IV - RE knockout primer 2) 1st PCR step - in 2 separate tubes, perform a PCR using the following set of oligos: oligos I and IV oligos II and III 3) Mix the PCR products from the first step (1 µl each) and use
Inverse PCR & Cycle Sequencing
) 2.0ul 1) Incubate O/N at 4°. 2) Ethanol precipitate on ice, 10min. 3) Spin, aspirate, pulse, aspirate. 4) Speed Vac briefly to dry pellet. 5) Resuspend in 150ul TE (~1/150 fly per ul). IV. PCR Set up PCR reactions
Salmonella Phages and ProphagesGenomics and Practical Aspects
include Gifsy-1, Gifsy-2, and Fels-1. The P22-like viruses include ɛ34, ES18, P22, ST104, and ST64T. The only member of the T7-like group is SP6. The properties of each of these phages are discussed, along with their role as agents of genetic exchange
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