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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 mM EDTA,50% Ethanol 6 M NaI note
DNA Extraction Protocols Using Silica
hood using PCR-free chemicals and supplies.Materials needed (suggested stocks are sufficient for several hundred "extractions"): Silica - ~100 g, Sigma # S-5631 GuSCN - ~500 g, Amresco (cheaper) # 0380 or Sigma # G-6639 Triton X-100
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