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文献和实验In Vitro Expansion of Tissue Cells by Conditional Proliferation
, purification, manipulation, or cloning. Here, we describe a method that allows the reversible expansion of cells by the introduction of a proliferator gene controlled by a regulatable expression module. The module is transferred by DNA transfer or by lentiviral
with transfer module--prefill to about 2/3 with of cold transfer buffer (For the biorad system,you use the same tank that you ran your gels in,but with a red and black transfer module that lives in the drawer (gel room,third drawer under the hybridization oven
BiSearch: ePCR Tool for Native or Bisulfite-Treated Genomic Template
can be repeat elements, whereas complex templates are targets for mispriming and alternative amplification products. Such examples are GC-rich native or bisulfite-treated genomic DNA sequences. Bisulfite treatment leads to the specific conversion of non
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