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文献和实验色谱柱和色谱系统的故障检修HPLC Column and System Troubleshooting
填料 5.Determining the Cause and Correcting High Back Pressure 查出原因 纠正压力 Check pressure with/without column - many pressure problems are due to blockages in the system or guard 有/无柱子时检查压力 - 许多压力问题是由于系统或保护柱的阻塞 If Column Pressure
, Norwalk, CT) used as an extraction column; a Nova-Pak C18 analytical column (2.1 � 150 mm I.D., 4 μm, Waters Corp, Milford, MA); washing solution, methanol: 30 mM ammonium acetate pH 5.1 (80:20); eluting solution, methanol:30 mM ammonium acetate pH 5.1 (97
Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
of the signals depends of course on the amount injected onto the column. In capillary GLC, only very small volumes can be carried through the column. Since it is impractical to inject 1/20 of a microliter, for example, our instrument uses a split injection mode
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