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文献和实验Vital Staining of Chromosomes with Hoechst 33258
with regular medium. 5. Add regular medium to the dish. Fluorescence can be observed with Zeiss 02 UV filter set and quartz-halogen lamp. Mercury arc lamps yield a much higher intensity but could cause damange to live cells. Note: the same protocol
Use of HMS Genetics Confocal Microscope
startup • Turn on the scope (switch on base, toward back, on the right). • Turn on the mercury lamp for the scope (small white box to the left of the scope). • Turn on the green laser (far right
m/pixel. The maximum image size is 1320x1035x12 bit. The 100 W mercury lamp and the diaphragms of the microscope were precisely adjusted to get a homogeneous illumination of the optical field. For each metaphase spread three monochrome images
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