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文献和实验An ELISA Lab-on-a-Chip (ELISA-LOC)
(ELISA-LOC) by integrating the washing step directly into an ELISA plate. LOM has been shown to be capable of creating complex 3D microfluidics through the assembly of a stack of polymer sheets with features generated by laser micromachining
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
kidney) and sFRP-2/MDCK clonally derived, transfectant cell lines. 2. Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO;Invitrogen), with and without 10% (v/v) fetal calf serum(Biosource; Invitrogen). 3. Phosphate-buffered saline (PBS): 1.54 mM KH
Synthesis of Peptide‐Oligonucleotide Conjugates Using a Heterobifunctional Crosslinker
in the assembly of bivalent protein affinity reagents. This unit describes the synthesis and purification of POC molecules using the heterobifunctional crosslinking reagent succinimidyl?4?(N?maleimidomethyl)cyclohexane?1?carboxylate (SMCC), which enables amine
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