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文献和实验运用肽库筛选磷酸化激酶motif方法 Screening Kinase Phosphorylation Motifs Using Peptide Libraries
To make the IMACcolumn: Clamp a 3-ml disposable chromatography column in a ring stand, keeping the tip plugged. Fill the column with 2-3 ml of water, then add 900 µl of a 1:1 slurry of the IDA agarose, and allow the resin to settle
is separated from the DNA by loading the solution onto an equilibrated ion exchange column. The A260 containing fractions are pooled, diluted, and ethanol precipitated, and the final DNA pellet is resuspended in buffer and assayed by restriction digestion
and then rinsed with ddH2O). 5. Insert the well forming comb, clamp, and allow the gel to polymerize for at least 30 minutes. 6. Prior to loading, remove the tape around the bottom of the gel and the well-forming comb. Assemble the vertical electrophoresis
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